The experiment based on its jib clam take blood smear blood cells, according to the characteristics of morphology, its jib clam blood into transparent cells, cells, and fibroblasts, according to the size of particles cells were divided into small granulosa cells, big granulosa cells, according to its moisturecontent again into eosinophilic granular cells and basophilic granulosa cells. In M199 medium and RPMI1640 medium as the foundation, by using different culture conditions, the cultivation of blood on, the results show that, based on M199 medium, 20% of the tire bovine serum, 5% of 1 x Hank 's balance salt, 100ug/mL double antioxidant and the net, 28-30 degrees Celsius temperature, pH value under the conditions of the 7.0 ~ 7.2 training effect is good, cultivate 24h after mitotic speed, 20% of the cells, the cell growth can maintain shapes, 7 days or so. Cells suspended 80%, the cell vitality, cultivate 14 days, still can spread to the second generation.
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Through this experiment, blood Hyriopsis cumingii blood smear observation, based on blood cell morphology characteristics, will be Hyriopsis cumingii blood cells are divided into clear cell, granular cells and fibroblasts, in which granulosa cells are divided into the basis of their small size granulosa cells and large granular cells, in accordance with its Dyeability particles are divided into eosinophilic cells and basophilic granular cells. To M199 medium and RPMI1640 medium, based on the use of different culture conditions, the blood cells of primary culture, the results showed that the M199-based medium, add 20% fetal bovine serum, 5% 1 × Hank `s salt balance, 100ug/mL and support of anti-double the original net, temperature 28 ~ 30 ℃, pH value of 7.0 ~ 7.2 under the conditions of the culture better, after 24h culture accelerated cell division, 20% of the adherent cell growth, paste parietal cells of different shape, growth can be maintained for about seven days. 80% of suspended cells, cell viability, and training 14 days later, still reached the second generation.