假设把ListBox1中全部的值传到ListBox2中—— for (int i = 0; i < listBox1.Items.Count; i++) { listBox2.Items.Add(listBox1.Items[i]); listBox1.Items.RemoveAt(i); i--; } 假设只是部分选中的: 1)首先把ListBox1的SelectionMode设置成MultiSimple. 2)运行程序,多选几个Item,然后: for (int i = 0; i < listBox1.SelectedItems.Count; i++) { listBox2.Items.Add(listBox1.SelectedItems[i]); listBox1.Items.RemoveAt(listBox1.FindString(listBox1.SelectedItems[i].ToString())); i--; }