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Objective: To study the First Hospital of Ningbo City in 2007 to 2009 the frequency of antimicrobial drugs in rooms with micro-organisms resistant Klebsiella pneumoniae culture correlation between the degree of change. Methods: The calculation of 2007 to 2009, Ningbo City, a quinolone hospital an average of 100 beds per day consumed DDDs (DDDs), and during this period the rate of resistance of Klebsiella pneumoniae, and two of the the relationship between the correlation analysis. Results: The three years to six months as a phase, Levofloxacin DDDs (in DDDs as a unit) from the first half of 2007 from 0.667 to 2008 in the first half to reach the highest 0.887, and then gradually decline; Cyclopropane sand Star DDDs from the first half of 2007 from 0.359 to 2008 to reach the highest 0.403 in the first half, and then gradually decreased. At the same time, measured by the laboratory Klebsiella pneumoniae levofloxacin resistance rate from 47.86% in the first half of 2007 rose to 67.40 percent in the first half of 2008, to return to the second half of 2009 fell 44.10% ; on the rate of ciprofloxacin resistance of about 50.83 percent in the first half of 2007 rose to 68.09 percent in the first half of 2008, to return to the second half of 2009 to 45.82%. Conclusion: The three years of Klebsiella pneumoniae to Fluoroquinolones in antimicrobial drug resistance of higher and higher as the DDDs, and decreases with the decrease in DDDs. Correlation existed between the two prompted clinicians should strictly control the indications for the use of quinolone antibiotics, empirical medicine must continue to refer to the Court of changes in bacterial resistance to antibiotics, the most in the least to play the role of quinolone antibiotics.