PCR和LAMP的电泳条带有什么区别

2025-04-07 03:58:23
推荐回答(1个)
回答1:

这两个方法我感觉原理还差挺多的,
lamp,环介导等温扩增法,英文名称为loop-mediatedisothermal
amplification,,是一种新型的核酸扩增方法,其特点是针对靶基因的6个区域设计4种特异引物,在链置换dna聚合酶(bst
dna
polymerase)的作用下,60--65℃恒温扩增,15-60分钟左右即可实现10^9~10^10倍的核酸扩增
pcr技术的基本原理类似于dna的天然复制过程,其特异性依赖于与靶序列两端互补的寡核苷酸引物。pcr由变性--退火--延伸三个基本反应步骤构成:①模板dna的变性:模板dna经加热至93℃左右一定时间后,使模板dna双链或经pcr扩增形成的双链dna解离,使之成为单链,以便它与引物结合,为下轮反应作准备;②模板dna与引物的退火(复性):模板dna经加热变性成单链后,温度降至55℃左右,引物与模板dna单链的互补序列配对结合;③引物的延伸:dna模板--引物结合物在taqdna聚合酶的作用下,以dntp为反应原料,靶序列为模板,按碱基互补配对与半保留复制原理,合成一条新的与模板dna链互补的半保留复制链,重复循环变性--退火--延伸三过程就可获得更多的“半保留复制链”,而且这种新链又可成为下次循环的模板。每完成一个循环需2~4分钟,2~3小时就能将待扩目的基因扩增放大几百万倍。
两者的酶,引物的设计,扩增的流程,产物的检验均不同,所以应该关系不大吧。而且虽然lamp相对简便一些,但两者还是各有利弊的。
希望对你有帮助,望采纳

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