考马斯亮蓝G-250和考马斯亮蓝R-250的区别？

2025-03-26 04:21:52

推荐回答（2个）

回答1：

属于三苯甲烷类染料，可与蛋白质形成较强的非共价复合体。考马斯亮蓝R250多用于聚丙烯酰胺凝胶电泳后蛋白质条带的染色；考马斯亮蓝G250是布拉德福德(Bradford)蛋白质定量试剂的主要成分。

回答2：

非常详细的描述（WIKI）
Coomassie Brilliant Blue R-250 was first used to visualise proteins in 1963 by Fazekas de St. Groth and colleagues.[9] Protein samples were separated electrophoretically on a cellulose acetate sheet. The sheet was then soaked in sulfosalicylic acid to fix the protein bands and then transferred to a solution of the dye.

Two years later in 1965 Meyer and Lambert used Coomassie Brilliant Blue R-250 to stain protein samples after electrophoretic separation in a polyacrylamide gel.[10] They soaked the gel in a dye solution containing methanol, acetic acid and water. As the dye stained the polyacrylamide gel as well as the protein, to visualise the protein bands they needed to destain the gel which they did electrophoretically. Subsequent publications reported that polyacrylamide gels could be successfully destained using an acetic acid solution.

The first report of the use of the "G" form of the dye to visualise protein bands in polyacrylamide gels came in 1967, where the dye was dissolved in an acetic acid solution containing methanol.[11] In was subsequently discovered that the protein bands could be stained without staining the polyacrylamide by using a colloid of the "G" form of the dye in a trichloroacetic acid solution containing no methanol. Using this procedure it was no longer necessary to destain the gel.[12] Modern formulations typically use a colloid of the "G" form of dye in a solution containing phosphoric acid, ethanol (or methanol) and ammonium sulfate (or aluminium sulfate).[13][14][15][16]

The Bradford assay uses the spectral properties of Coomassie Brilliant Blue G-250 to estimate the amount of protein in a solution.[17] A protein sample is added to a solution of the dye in phosphoric acid and ethanol. Under the acid conditions the dye is normally a brownish colour but on binding to the protein the blue form of the dye is produced. The optical absorbance of the solution is measured at a wavelength of 595 nm.

On binding to a protein the negatively charged Coomassie Brilliant Blue G-250 dye molecule will give an overall negative charge to the protein. This property can be used to separate proteins or protein complexes using polyacrylamide gel electrophoresis under non-denaturing conditions in a technique called Blue Native PAGE.[18][19] The mobility of the complex in the polyacrylamide gel will depend on both the size of the protein complex (i.e. the molecular weight) and on the amount of dye bound to the protein.

最新问答

为什么适量的氯化钠溶于水后，水的体积不变？

在淘宝网上买的Win7系统U盘16G的(买回来直接就可以装系统的U盘)但装的系统不行，想把U盘格式

沙漠中的流沙是怎样形成的?

今天早上我干女儿出生了，姓王想大家帮着取个好听的名字

停车熄火关闭点火开关时，拧下钥匙车左门车哧的一声，怎么回事？

如何形容包装好？

问道里师门任务蓝色的布鞋

贵州大学全日制本科自考助学班专业招生有没有学前教育

如何用钉钉智能人事解决HR管理难题

打伞手势为什么侮辱人